Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil

Main Article Content

Andressa Sbano
Juliana B. Succar
João Victor R. Ferreira
Lidiane C. Berbert
Vinicius R. Flores
Janaína J. V. Cavalcante
Rodolpho M. Albano
Ida Carolina N. Direito
Alexander M. Cardoso

Abstract

Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil.

Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019.

Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST).

Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs.

Keywords:
Biofertilizers, biotechnology, inoculants, soil microbiota.

Article Details

How to Cite
Sbano, A., Succar, J. B., Ferreira, J. V. R., Berbert, L. C., Flores, V. R., Cavalcante, J. J. V., Albano, R. M., Direito, I. C. N., & Cardoso, A. M. (2020). Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil. Asian Journal of Biotechnology and Bioresource Technology, 6(1), 11-13. https://doi.org/10.9734/ajb2t/2020/v6i130071
Section
Data Article

References

Chong NM. Development of a tool for measuring the degradation capacity of microorganisms for a xenobiotic. Enzyme and Microbial Technology. 2005;37:467-471.

Strahsburger E, Zapata F, Pedroso I, Fuentes D, Tapia P, Ponce R, Valdes J. Draft Genome Sequence of Bacillus safensis RP10, Isolated from Soil in the Atacama Desert, Chile. Microbiology Resource Announcements. 2019;8(44).

Coil D, Jospin G, Darling AE. A5-miseq: An updated pipeline to assemble microbial genomes from Illumina MiSeq data. Bioinformatics. 2015;31:587-589.

Haft DH, DiCuccio M, Badretdin A, Brover V, Chetvernin V, O'Neill K, Li W, Chitsaz F, Derbyshire MK, Gonzales NR, Gwadz M, Lu F, Marchler GH, Song JS, Thanki N, Yamashita RA, Zheng C, Thibaud-Nissen F, Geer LY, Marchler-Bauer A, Pruitt KD. RefSeq: An update on prokaryotic genome annotation and curation. Nucleic Acids Res. 2018;46:851-860.
DOI: 10.1093/nar/gkx1068.

Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. The RAST server: Rapid annotations using subsystems technology. BMC Genomics. 2008;9:75.

Richter M, Rosselló-Móra R, Glöckner FO, Peplies J. J Species WS: A Web prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics. 2016;32:929–931.
DOI: 10.1093/bioinformatics/btv681

Moreira V, Cardoso AM. As Superbactérias Resistentes a Antibióticos Estão Chegando?. Acta Scientiae et Technicae. 2016;4:144-147.
DOI: 10.17648/uezo-ast-v4i2.144

Cardoso AM, Cavalcante JJV, Cantão ME, Thompson CE, Flatschart RB, Glogauer A, Scapin SMN, Sade YB, Beltrão PJMSI, Gerber AL, Martins OB, Garcia ES, Souza W, Vasconcelos ATR. Metagenomic Analysis of the microbiota from the crop of an invasive snail reveals a rich reservoir of novel genes. PLoS ONE. 2012;7: e48505.
DOI: 10.1371/journal.pone.0048505