Asian Journal of Biotechnology and Bioresource Technology http://www.journalajb2t.com/index.php/AJB2T <p style="text-align: justify;"><strong>Asian&nbsp;Journal of Biotechnology and Bioresource Technology (ISSN:&nbsp;2457-0125)&nbsp;</strong>aims to publish&nbsp;high quality papers (<a href="/index.php/AJB2T/general-guideline-for-authors">Click here for Types of paper</a>)&nbsp;in all areas of Biotechnology and Bioresource Technology. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.&nbsp;</p> en-US contact@journalajb2t.com (Asian Journal of Biotechnology and Bioresource Technology) contact@journalajb2t.com (Asian Journal of Biotechnology and Bioresource Technology) Fri, 15 Nov 2019 08:24:51 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Comparison of Mycelial Growth of Different Tricholoma matsutake Strains in Soil Medium at Varying Temperatures http://www.journalajb2t.com/index.php/AJB2T/article/view/30066 <p><strong>Aims: </strong>To investigate the effect of temperature on the mycelial growth of the highly prized mushroom, <em>Tricholoma matsutake</em>, in soil medium.</p> <p><strong>Methodology:</strong> Seven strains of <em>T. matsutake</em> collected from different areas of Japan were incubated in soil medium at 5, 10, 15, 20, 25, and 30°C for 89 day. After incubation, the linear growth of mycelia from the inoculum was measured on vertical lines under a dissecting microscope and classified by the cultivation day number to obtain the growth rate in each vial. The mycelial densities in the soil medium in all the vials were analyzed using qPCR to determine the mycelial biomass.</p> <p><strong>Results:</strong> The mycelial growth of <em>T. matsutake</em> strains was mainly observed at 5–25°C. The optimum temperatures for linear mycelial growth and for the increase in mycelial density were 19.6°C and 17.6°C, respectively. These values were lower than those reported previously for <em>T. matsutake</em> grown on agar medium or in liquid medium. These optimum temperatures showed no clear relationship with the mean air temperature at the site of strain collection.</p> <p><strong>Conclusion:</strong> It can be suggested that a temperature around 18°C is suitable for cultivation of <em>T. matsutake</em> mycelia in the soil medium.</p> Maki Narimatsu, Muneyoshi Yamaguchi, Takashi Yamanaka, Seiki Gisusi, Tomonori Azuma, Yutaka Tamai, Tohru Fujita, Masataka Kawai ##submission.copyrightStatement## http://www.journalajb2t.com/index.php/AJB2T/article/view/30066 Wed, 20 Nov 2019 00:00:00 +0000 Assessment of Inbreeding Depression and Inbreeding Trend of Production Traits in Iraqi Holstein Cows http://www.journalajb2t.com/index.php/AJB2T/article/view/30064 <p>Inbreeding is defined as the probability that two alleles at any locus are identical by descent and occur when related individuals are mated to each other. A total of 123427, 115810 and 88361 records of 412-d yields of milk, fat and protein of Iraqi Holstein cows were collected from 1995 to 2010 in 838 herds used to estimation the inbreeding depression and inbreeding trend. Pedigree records of Iraqi Holstein cow were used to assessment inbreeding coefficients and these coefficients ranged from 0 to 42%. Animal model was used to estimation inbreeding depression on traits. Fixed effects included in statistical model were herd – year, age at calving and inbreeding coefficient as continuous and discrete variable. When considering inbreeding as continuous variable in model, the inbreeding depression for 412-d yields of milk, fat and protein were -28.19, -0.98 and -0.88 kg per 1% increase in inbreeding in Iraqi Holsteins, respectively<strong>. </strong>In this group of animal that inbreeding coefficient was between 0 &lt; F ≤ 5.34 inbreeding was not caused reduction in production traits. However, in group of animal that inbreeding coefficient was greater than 5.34, and inbreeding depression in production traits was observed. The result of this study confirms of inbreeding depression in Iraqi Holstein cows.</p> Farzad Moradpour, Hana Hamidi ##submission.copyrightStatement## http://www.journalajb2t.com/index.php/AJB2T/article/view/30064 Fri, 15 Nov 2019 00:00:00 +0000 Optimization of Aspergillus niger α-amylase Activity for Enhanced Glucose Production from Cassava Starch http://www.journalajb2t.com/index.php/AJB2T/article/view/30065 <p>The aim of this study was to optimize the hydrolytic activity of<em> A. niger</em> α-amylase on cassava starch. Isolation of <em>Aspergillus niger,</em> determination of α-amylase activity, α-amylase production and extraction were performed using standard protocols. Parameters such as pH, temperature, substrate concentration were studied using unifactorial approach. pH was varied from 3.6-5.6, temperature 30-80ºC, substrate concentration 0.3-1.5 g/l. In conclusion, for optimal utilization of α-amylase in the production of numerous products of economic significance, the outcome of this work can be relied upon to boost production of glucose and accessory products from starch.</p> N. A. Osaribie, J. C. Ndubuisi, O. Ewa, M. R. Karimah, O. Oda ##submission.copyrightStatement## http://www.journalajb2t.com/index.php/AJB2T/article/view/30065 Mon, 18 Nov 2019 00:00:00 +0000 The Ethanolic Extracts of the Root, Stem and Leaves of Balanites aegyptiaca against Selected Microbes http://www.journalajb2t.com/index.php/AJB2T/article/view/30067 <p><em>Balanites aegyptiaca</em> has been used in many traditional treatments of microbial infections. This work was carried out with the aim of determining the phytochemical compounds present in the ethanolic extracts of the root, stem and leaves of <em>Balanites aegyptiaca</em> and their effects against selected microorganisms. Phytochemical screening was undertaken to determine presence of secondary metabolites in the stem-bark, roots and leaves of the test plant. Well diffusion method was used to determine sensitivity of test organisms to the test plant. Result indicated the presence of alkaloids, tannins, flavonoids, saponins, steroids, and anthraquinones, although anthraquinones were absent in ethanolic extracts of root and stem bark of <em>Balanites aegyptiaca </em>but present in the ethanolic extract of the leaf. Antibacterial effects were tested against <em>Staphylococcus aureus </em>and <em>Klebsiella pneumoniae</em>. Results obtained from the ethanolic extract of the root stem and leaves showed significant zones of inhibition against <em>Klebsiella pneumoniae </em>and <em>Staphylococcus aureus </em>at all concentrations of 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.125 mg/ml. The positive control performed better than the extracts in terms of zone of inhibition. The results of the minimum inhibitory concentration (MIC) indicated that the leaf ethanolic extract had the greatest activity against<em> K. pneumoniae </em>with MIC value of 12.5 mg/ml, while the results from the minimum bactericidal concentration (MBC) showed that the leaf ethanolic extract had the greatest activity against <em>K. pneumoniae</em> with an MBC value of 12.5 mg/ml.</p> D. Kulawe, N. Ahidjo, J. S. Hena ##submission.copyrightStatement## http://www.journalajb2t.com/index.php/AJB2T/article/view/30067 Thu, 21 Nov 2019 00:00:00 +0000