Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil
Andressa Sbano
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil and Division of Metrology Applied to Life Sciences, National Institute of Metrology, Quality and Technology - INMETRO, Rio de Janeiro, Brazil.
Juliana B. Succar
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil.
João Victor R. Ferreira
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil and Division of Metrology Applied to Life Sciences, National Institute of Metrology, Quality and Technology - INMETRO, Rio de Janeiro, Brazil.
Lidiane C. Berbert
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil.
Vinicius R. Flores
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil and Division of Metrology Applied to Life Sciences, National Institute of Metrology, Quality and Technology - INMETRO, Rio de Janeiro, Brazil.
Janaína J. V. Cavalcante
Division of Metrology Applied to Life Sciences, National Institute of Metrology, Quality and Technology - INMETRO, Rio de Janeiro, Brazil.
Rodolpho M. Albano
Department of Biochemistry, Institute of Biology Roberto Alcântara Gomes, State University of Rio de Janeiro - UERJ, Rio de Janeiro, Brazil.
Ida Carolina N. Direito
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil.
Alexander M. Cardoso *
Laboratory of Environmental Biotechnology, Western Rio Janeiro State University - UEZO, Rio de Janeiro, Brazil and Division of Metrology Applied to Life Sciences, National Institute of Metrology, Quality and Technology - INMETRO, Rio de Janeiro, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil.
Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019.
Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST).
Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs.
Keywords: Biofertilizers, biotechnology, inoculants, soil microbiota.