Response Surface Optimization of Extraction of Polyphenols and Carotenoids from Sesbania grandiflora Leaves with Ethanol-water System
K. D. P. P. Gunathilake *
Department of Food Science and Technology, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, Sri Lanka
K. K. D. S. Ranaweera
Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
H. P. V. Rupasinghe
Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada
O. D. A. N. Perera
Department of Food Science and Technology, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, Sri Lanka
H. P. S. Jayaweera
Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, Sri Lanka
*Author to whom correspondence should be addressed.
Abstract
Response Surface Optimization of Extraction of Polyphenols and Carotenoids from Sesbania grandiflora Leaves with Ethanol-water System
Aim: Response surface methodology was employed to optimize the extraction parameters for the extraction of total phenolics and carotenoids from leaves of Sesbania grandiflora with ethanol-water based system.
Method: The effects of solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time (30-90 min) on the recovery of total phenolics and carotenoids were investigated. Results and Discussion: The constructed models were adequate to explain the behavior of the extraction system and predict the responses, total phenolics (R2 = 88.53%) and carotenoid (R2 = 90.60) contents. The optimum extraction conditions of ethanol concentration, extraction temperature and extraction time for phenolics, were 46.6%, 70.2°C, and 110.5 min and for carotenoids, the optimum parameters were 100%, 70.2°C and 110.5 min, respectively. The optimal predicted contents for total phenolics (7.74 mg Gallic Acid Equivalent (GAE)/ g DW) and carotenoids (4.32 mg/g DW) values in the extracts were agreed with the experimental values obtained with optimum extraction conditions for each response.
Keywords: Sesbania grandiflora leaves, phenolics, carotenoids, response surface methodology