https://journalajb2t.com/index.php/AJB2T/issue/feedAsian Journal of Biotechnology and Bioresource Technology2024-03-26T11:32:15+00:00Asian Journal of Biotechnology and Bioresource Technologycontact@journalajb2t.comOpen Journal Systems<p><strong>Asian Journal of Biotechnology and Bioresource Technology (ISSN: 2457-0125)</strong> aims to publish high quality papers (<a href="https://journalajb2t.com/index.php/AJB2T/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of Biotechnology and Bioresource Technology. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p><strong>NAAS Score: 4.66 (2024)</strong></p>https://journalajb2t.com/index.php/AJB2T/article/view/200Effects of Commercial Feeding with Different Proteins on the Growth of G6 Transgenic Mutiara Catfish in Budikdamber Rearing2024-03-23T07:58:04+00:00Ibnu D. Buwono0812ibnu@gmail.comRoffi G.HKiki HAhmad A.R<p>This research was conducted in the Hatchery Building 4 Faculty of Fisheries and Marine Science, Padjadjaran University. The implementation started from July to September 2023. The purpose of this study was to test the growth performance of G6 transgenic mutiara catfish by feeding commercial feed containing different proteins to induce growth rate and protein use efficiency. The treatment design used the complete randomized design (CRD) method with four treatments (A: 39% feed protein; B: 32% feed protein; C: 20% feed protein; and D: 37% feed protein (mixture of pindang tongkol and PF.1000)) with three replications. Data were analysed using Sigmaplot 15.0 for absolute weight gain (Wg), feed conversion ratio (FCR), protein retention (RP), and protein productive value (PPV) and using Duncan Multiple Range Test (DMRT) with 95% confidence level. The results showed that treatment A gave the best results on the value of Wg (average weight gain of 4619 g ± 344.3 g) and the lowest FCR (0.63 ± 0.058), and the best protein retention and PPV values were shown in treatment B, as follows 19.24% and 17.4%.</p>2024-03-23T00:00:00+00:00Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://journalajb2t.com/index.php/AJB2T/article/view/201Identification of Antibiotics Susceptibility Patterns at Sub-inhibitory Concentrations in Pseudomonas aeruginosa2024-03-26T11:32:15+00:00C. C. Williamsenyenwaniwilliams@gmail.comG. A. AjibadeV. M. Y. Dan<p><em>Pseudomonas aeruginosa </em>is a Gram negative bacterium that has been recognized as an opportunistic pathogen. It is the most common bacterium associated with nosocomial infections and ventilator-associated pneumonia. It exhibits high innate resistance to various ranges of antibiotics thereby causing high morbidity and mortality rate. This research was aimed to identify the antibiotics susceptibility patterns at sub-inhibitory concentration in <em>Pseudomonas aeruginosa</em>. One hundred and fifty (150) clinical swab specimens were collected from urinary catheters; wound and ear infections of patients and the swabs inoculated using standard microbiology method. The isolates were characterized based on the bacteriological methods such as morphology and biochemical tests. The isolates were further confirmed by species specific by PCR amplification of 16S rRNA and the amplicons were analyzed by gel electrophoresis; and further genomic sequencing was done and blast with NCBI database mining. The antimicrobial susceptibility of the isolates was done by disc diffusion methods. The result of the isolation showed 22(59.46%) from wound infections, 12(32.43%) from ear infections and 3(8.11%) from urinary catheter. The isolates were Gram-negative, produced β-hemolysis on blood agar and the morphology is small pigmented circular. The isolates showed positive results to catalase, oxidase, citrate, nitrate and indole tests. The amplification of the 16S rRNA gene region resulted in the band size of 1500bp PCR product and the BLAST analysis gave 99% similarity. The results of susceptibility analysis showed that the isolates from the urinary catheter, wound and ear infection were 82%, 68% and 47% respectively, resistance to Piperacillin tazobactem, Cefoperazon, Ofloxacin, Tetracycline, Amikacin, Gentamycin, Bacitracin, Clarithromycin, Cefalotin, Levofloxacin and Cefpiroma. Antimicrobial susceptibility tests in <em>P. aeruginosa</em> isolates revealedshowed that they were multi-drug resistant. At sub-inhibitory concentrations of antibiotics within the microbial environment, <em>Pseudomonas aeruginosa</em> becomes more resistant. Perhaps, these antimicrobials could have other signaling activities within the environment. Therefore, there is a need for more research work to develop therapeutics combination to combat the recalcitrant nature of <em>Pseudomonas aeruginosa.</em></p>2024-03-26T00:00:00+00:00Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.