Asian Journal of Biotechnology and Bioresource Technology https://www.journalajb2t.com/index.php/AJB2T <p><strong>Asian Journal of Biotechnology and Bioresource Technology (ISSN: 2457-0125)</strong> aims to publish high quality papers (<a href="https://journalajb2t.com/index.php/AJB2T/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of Biotechnology and Bioresource Technology. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p><strong>NAAS Score: 4.66 (2026)</strong></p> SCIENCEDOMAIN international en-US Asian Journal of Biotechnology and Bioresource Technology 2457-0125 Biotechnological Innovations for Unlocking the Antimicrobial Potential of Endophytic Fungal Metabolites https://www.journalajb2t.com/index.php/AJB2T/article/view/288 <p>Antibiotic consumption has augmented globally without proper guidelines, accelerating the development of bacterial resistance to them, which is the ability of microorganisms to survive and proliferate despite the action of these substances. Given this situation, it is crucial to seek more accessible alternatives, such as endophytic fungi. These are eukaryotes that inhabit the internal tissues of plants, where they establish mutually beneficial symbiotic relationships without harming them. Research to date has demonstrated that the genetic diversity and variety of secondary metabolites found in these fungi reinforce the importance of continuing to explore them, as certain molecules have shown antibiotic activity against various pathogenic bacteria. Nonetheless, their effective utilization is limited by their low natural productivity and the lack of knowledge about their symbiotic relationships and the regulation of their metabolic pathways. Therefore, biotechnological strategies have been developed and implemented to overcome these limitations. Techniques such as media optimization, the one strain many compounds (OSMAC) approach, co-culture, epigenetic modification, and genetic engineering have been used to activate latent biosynthetic pathways and increase metabolite production. However, research has not included animal models, much less clinical trials. Therefore, the path to commercialization still requires significant research by the pharmaceutical industry.</p> María Daniela Castro-Herrera Jose Alejandro Chaves-Gómez Ericka María Núñez-Rojas Alejandro Portuguez-Blanco Yanitza Valeria Torres-Alvarado Juan José Mora-Román Copyright (c) 2026 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2026-03-13 2026-03-13 12 2 1 20 10.9734/ajb2t/2026/v12i2288 Molecular Authentication and Phylogenetic Characterization of Curcuma aeruginosa Roxb. (Zingiberaceae) Using Chloroplast rbcL Gene Sequences https://www.journalajb2t.com/index.php/AJB2T/article/view/289 <p><em>Curcuma aeruginosa</em> Roxb. (Zingiberaceae) is a medicinally significant species frequently confused with morphologically allied taxa such as <em>C. caesia</em> and <em>C. zedoaria</em> in the herbal trade. This study authenticates <em>C. aeruginosa</em> collected from Assam, India, using an integrative taxonomic framework through molecular, morphological and herbarium-based approaches.</p> <p><em>Ex-situ </em>morphological validation revealed erect pseudostems with oblong-lanceolate leaves bearing reddish midribs, terminal inflorescences with pink bracts and bluish-violet rhizomes, which are diagnostic features consistent with<em> C. aeruginosa</em>. The specimen was taxonomically verified at the Botanical Survey of India, Coimbatore (Voucher No. BSI/SRC/5/23/2023/Tech–875). The <em>rbcL</em> gene was successfully amplified (564 bp) and sequenced (GenBank accession PQ287241). BLAST analysis showed 97% query cover and 96.15% identity with <em>C. aeruginosa</em> accessions (MK934697.1, KX758482.1), confirming its molecular identity. Phylogenetic analysis positioned the specimen within a distinct <em>C. aeruginosa</em> clade with strong bootstrap support (99%). This integrative study establishes a verified reference for <em>C. aeruginosa</em> within the Indian flora, reinforces its taxonomic distinctiveness, and highlights the importance of DNA barcoding for resolving identification ambiguities in <em>Curcuma</em> and related Zingiberaceae taxa. The study also highlights the practical importance of molecular authentication for ensuring correct identification of medicinal <em>Curcuma </em>species in herbal markets and pharmacognostic research.</p> S. Narthanaa M. A. Deepa M. Pugalenthi Copyright (c) 2026 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2026-03-24 2026-03-24 12 2 21 29 10.9734/ajb2t/2026/v12i2289